Lab Members

Principal Investigator

Daniel Wagenaar

daw@caltech.edu / +1 (626) 395-8567

Daniel Wagenaar obtained his PhD in Physics from Caltech studying the networks formed by cultures of rat cortical neurons grown on multi-electrode arrays working with Jerry Pine and Steve Potter (at Georgia Tech). Before that, he studied theoretical physics as an undergraduate student at the University of Amsterdam and information theory and neural networks as a Master’s student in the Math Department of King’s College, London. (More...)

After his PhD, Dr Wagenaar worked as a postdoc in the lab of Bill Kristan at UCSD, where he combined electrophysiology and voltage-sensitive dye imaging to study motor behavior in the medicinal leech, and in 2008, he started his own research group at Caltech as a Broad Senior Research Fellow where he started his work on crossmodal sensory integration. (More...)

From September 2012 through July 2013, Dr Wagenaar spent a year as a Senior Research Fellow in the new lab of Markus Meister at Caltech working on the design and construction of two-photon microscopes.

From August 2013 through July 2016, Dr Wagenaar worked at the Department of Biological Sciences at the University of Cincinnati as an Assistant Professor. Now, Dr Wagenaar is taking his lab back at Caltech, as a Research Professor with responsibility for the newly founded Neurotechnology Center.

Postdocs

Mária Ashaber, 2016–present

ashaberm@caltech.edu

Mária Ashaber received her Ph.D. from the Semmelweis University School of Ph.D. Studies (Faculty of Neuromorphology) in Budapest working in the lab of Dr László Négyessy. She is currently working on electron microscope imaging and 3D reconstruction of neuronal networks in the leech.

Angela Bruno, 2016–present

abruno@caltech.edu

Angela Bruno received her Ph.D. in Neuroscience from Rosalind Franklin University, The Chicago Medical School in North Chicago, IL. She worked in the lab of William Frost, and was co-mentored by Dr. Mark Humphries at the University of Manchester.

Funded by a pre-doctoral fellowship from the NIH, her work led to an unsupervised approach to mapping neural networks (Neuron 2015), and to the characterization of the first observed spiral attractor in a biological system (bioRxiv 2017). Before turning to systems neuroscience, she utilized her B.S. in Biochemistry from the University of Illinois to co-author numerous publications in approaches to Alzheimer’s disease.

Dr. Bruno also attended three summer courses at The Marine Biological Laboratory in Woods Hole; Neural Systems & Behavior, Methods in Computational Neuroscience, and Brains, Minds, & Machines. After learning the different perspectives and approaches to understanding the brain, Dr. Bruno’s primary interest is to identify fundamental principles that describe how circuit connectivity and neuronal population dynamics give rise to behavior. It is this interest that led her to join the Wagenaar Lab in September 2016.

Yusuke Tomina, 2014–present

tominaye@caltech.edu

Yusuke Tomina obtained his PhD in Life Science from Hokkaido University (Sapporo, Japan) in March, 2014. He carried out a series of behavioral and physiological studies of “goal-directed” gripping behavior in American lobster, working in the lab of Masakazu Takahata as a JSPS fellowship researcher (April, 2011-March, 2014). He worked as a neuroethologist with decapod crustaceans (lobster and crayfish) from undergraduate to post-doc in Hokkaido University. Dr Tomina joined the Wagenaar lab as a postdoc in August 2014.

Dr Tomina is interested in how we can understand animal behavior and its perception as a function of the nervous system and also likes the philosophy of “Krogh’s principle”. His primary research motivation is to describe animals’ perceptual world (referred to as "Umwelt") as internal neuronal processes by using physiological terms especially at the level of single cells and their network. He is also curious about phylogenic evolution of neuronal systems responsible for the broad aspects of animal behavior including perception, motor control and also cognition. He considers that, from the point of view of comparative biology, we neurobiologists are required to think about “which findings are likely to be biologically general and which are likely to be distinctive of a species” (as Eve Marder wrote in “Invertebrate Neurobiology”, 2007).

Alumni

Annette Stowasser, postdoc, 2013–2016

stowasa@uc.edu

Dr Stowasser obtained her PhD from the University of Cincinnati in 2013 studying the optical system of the principal eyes of the diving beetle Thermonectus mamoratus in Elke Buschbeck’s lab. She is currently working on neuronal pathways of visual processing in the midbody ganglia of the medicinal leech.

Andrew (Drew) Lehmkuhl, graduate student, 2015–2016

lehmkuaw@mail.uc.edu

Drew Lehmkuhl earned his Bachelors in Neuroscience from the University of Cincinnati in 2013. As an undergraduate student, he worked with Dr. Bruce Jayne, exploring how branch structure affects gap-bridging behaviors in brown treesnakes. Drew's research interests include organism–environment interaction and behavioral neuroscience. His research in the Wagenaar Lab involved mechanoreception related to water disturbance in the medicinal leech. He obtained his M.S. in the summer of 2016 and will join the law school at UC in the fall.

Arunkumar Muthusamy, graduate student, 2014–2016

muthusar@mail.uc.edu

Arun Muthusamy completed his Bachelors in Chemistry and Masters in Biology at IISER-Thiruvananthapuram, India (2009-2014). Under the guidance of Dr. Tapas K. Manna, he studied how primary cilia assembly is effected by TACC proteins in RPE-1 cells. During the summer of 2013, he carried out a short-term project in the lab of Dr. Sanjay Sane at NCBS, Bangalore on how termites of the species Odontotermes obesus rebuild their mound. The project was aimed at understanding what sensory cues they use to recognize the presence of damage to the mound. This project perked his interest in understanding how animals use different senses and how they go about integrating them to carry out day-to-day functions and the structures they use for doing so.

In the Wagenaar Lab, Mr. Muthusamy worked on intersegmental signaling of prey-relevant sensory information. He is currently finishing up a manuscript on this work and is joining the lab of Elke Buschbeck as a PhD student.

Daniel Hofmann, 2014–2015
Adam Stockfish, 2015
Chris Tandoc, 2014–2015

Undergraduate students Daniel ‘Dan’ Hofmann, Adam Stockfish, and Chris Tandoc used quantitative behavioral techniques to study the involvement of several sensory systems in prey localization.

Taylor Rupp, 2014

As an REU summer student in the lab, Taylor Rupp studied localization of visually perceived waves in the medicinal leech. She since joined Michigan State University as a graduate student.

Cynthia Harley, postdoc, 2009–2012

Cynthia ‘Cindy’ Harley is interested in how information from sensory systems translates into changes in motor behavior. She earned her PhD studying the involvement of the central complex, a region of the insect brain thought to be involved in sensory-motor integration, in obstacle negotiation behaviors working in the lab of Roy Ritzmann in the Biology Department at Case Western Reserve University. Working in my lab, she has discovered that leeches use visual and mechanical cues differentially, and that this difference is under developmental control. She also discovered that sensory information is used differently in crawling leeches than in swimming leeches.

After her work in my lab, Cindy went on to do a second postdoc in the lab of Karen Mesce at the University of Minnesota to trace the flow of visual information from the leech’s head eyes into the brain.

John Nagarah, postdoc, 2009–2012

John earned his Ph.D. in Physical Chemistry from UCLA and Caltech in 2009 under Prof. James R. Heath. For his thesis, he developed a batch fabrication process to create high performance planar patch-clamp wafers in quartz/glass and integrated them with multilayer PDMS microfluidic devices. In my lab, John Nagarah developed planar electrode technologies in quartz to simultaneously immobilize neural tissue, record extracellular signals, and image voltage sensitive dyes stained in neurons.

After his work in my lab, John took his expertise in microfabrication to Lam Research in Fremont, CA.

Pieter Laurens Baljon, visitor, 2010, 2011, 2012

Pieter Laurens Baljon graduated from the Neuroengineering group of Prof. Martinoia at the University of Genova, Italy. As a visitor to our lab in 2010, he worked on interfacing the leech ganglion with a 60-channel microelectrode array. Performing spike sorting and source separation on these recordings, one can follow a large number of cells at high temporal resolution, and for extended periods. With a simultaneous recording using voltage-sensitive dyes it becomes possible to link the electrophysiological signal to individual cells and their function. A year later (after accepting a postdoctoral position at the Center for Neurogenomics and Cognitive Research at the Vrije Universiteit Amsterdam) and again in 2012, he returned to our lab to study how a neural reflex pathway resolves sensory conflicts.

Matthew Rossi, high school student, 2011–2012

Matthew Rossi’s focus was on elucidating during which phases of prey localization behavior leeches actually take in sensory cues.

Javier Cienfuegos, high school student, 2010–2011

Javier Cienfuegos worked on prey localization experiments with Cindy Harley. We are proud to announce that he has since matriculated as an undergraduate student at Yale.

Andrew Krause, technician, 2009–2012